Cartoon depicting the stepwise procedure for peptide microarraying of an antibody from peptide immobilization to antibody hybridization and visualization. Positive interactions are visualized as red fluorescence. All printed spots show green fluorescence from the biotinylated fluorescein (5-FAM) printing control.
Raw scan of a histone peptide microarray hybridized with an H3K9me3 antibody (Diagenode C15410193). All printed peptides are shown as green spots. Antibodies bound to peptides are shown as red spots. IgG control spots are outlined in yellow. H3K9me3-containing peptides recognized by this antibody are outlined in red. Off-target peptides recognized by this antibody are outlined in white.